Inflammatory bowel disease (IBD) is induced by multiple environmental factors, and there\nis still no known treatment capable of curing the disease completely. We propose a zeolite-containing\nmixture (HydryeastÃ?®, HY)ââ?¬â?a multi-component nutraceutical of which the main ingredients are\nAzumaceramics (mixture of zeolite and oyster shell burned under high temperature), citric acid, red\nrice yeast (monascus) and calcium stearateââ?¬â?as a nutraceutical intervention in IBD to ameliorate\ndextran sodium sulfate (DSS)-induced colitis. We show the mechanism through integrated omics\nusing transcriptomics and proteomics. C57BL6 mice were given an AIN-93G basal diet or a 0.8%\nHY containing diet and sterilized tap water for 11 days. Colitis was then induced by 1.5% (w/v)\nDSS-containing water for 9 days. HY fed mice showed significantly improved disease activity index\nand colon length compared to DSS mice. Colonic mucosa microarray analysis plus RT-PCR results\nindicate HY supplementation may ameliorate inflammation by inhibiting the intestinal inflammatory\npathway and suppress apoptosis by curbing the expression of genes like tumor protein 53 and\nepidermal growth factor receptor and by upregulating epithelial protection-related proteins such as\nepithelial cell adhesion molecule and tenascin C, thus maintaining mucosal immune homeostasis\nand epithelial integrity, mirroring the proteome analysis results. HY appears to have a suppressive\neffect on colitis.
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